CHAPTER 1  INTRODUCTION TO THE PHARMACEUTIC ALANALYSIS
  1.1  Nature and Tasks of Pharmaceutical Analysis
    1.1.1  Pharmaceutical Analysis in the New Drug Discovery
    1.1.2  Pharmaceutical Analysis to Ensure the Quality of Drug Product
    1.1.3  Pharmaceutical Analysis in the Stability Studies
    1.1.4  In Vivo Drug Analysis
  1.2  National Standards for Supervision of Drug Quality
  1.3  Significance of Separation Technology in the Pharmaceutical Analysis
CHAPTER 2  CHROMATOGRAPHIC ANALYSIS
  2.1  Theoretical Principle and Parameters
    2.1.1  Theoretical Principles
    2.1.2  Separation Parameters
    2.1.3  Theoretical Plates of a Chromatographic Column
  2.2  Gas Chromatography
    2.2.1  Separation Mechanism
    2.2.2  GC Instrument
    2.2.3  GC Columns
    2.2.4  Stationary Phases in GC
    2.2.5  Stationary Phases in the GSC
    2.2.6  Stationary Phases in the GLC
    2.2.7  Detectors in GC
    2.2.8  Advantages of GC
    2.2.9  Applications of GC
  2.3  Introduction to Liquid Chromatography
    2.3.1  Columns in HPLC
    2.3.2  General Morphology of HPLC Stationary Phase Particles
  2.4  Normal Phase Liquid Chromatography
    2.4.1  Characteristics of Normal Phase Liquid Chromatography
    2.4.2  Elution Ability of Mobile Phase
    2.4.3  Applications
  2.5  Reversed-phase Liquid Chromatography
    2.5.1  Stationary Materials for Reversed-phase Liquid Chromatography
    2.5.2  Retention Characteristic of Reversed-phase Chromatography
    2.5.3  Secondary Chemical Equilibria in the Reversed-phase Chromatography
    2.5.4  Applications of Reversed-phase HPLC
    2.5.5  Ion-pair Reversed-phase Chromatography
  2.6  Ion-exchange Chromatography
    2.6.1  Separation Principle of IEC
    2.6.2  Stationary Phase Materials of IEC
    2.6.3  Retention of Analyses in IEC
    2.6.4  Major Factors in the IEC Separation
    2.6.5  Detection in IEC
    2.6.6  Advantages of IEC
    2.6.7  Application
  2.7  Size-exclusion Chromatography
    2.7.1  Separation Mechanism
    2.7.2  Packing Materials for SEC
    2.7.3  Applications of SEC
  2.8  Affinity Chromatography
    2.8.1  Nature of Affinity Interaction
    2.8.2  Separation Principle of Affinity Chromatography
    2.8.3  Ligand Selection
    2.8.4  Selection of Supporting Materials
    2.8.5  Preparation of Packing Materials for Affinity Chromatography
    2.8.6  Separation by Affinity Chromatography
    2.8.7  Application of Affinity Separation Technology
  2.9  Thin-layer Chromatography
    2.9.1  Stationary Phases Used in TLC
    2.9.2  Operation in TLC
    2.9.3  Performance Characteristics of TLC
    2.9.4  Detection in TLC
    2.9.5  Quantitative Analysis
    2.9.6  Applications of TLC Analysis
  2.10  Detectors in Liquid Chromatography
    2.10.1  Bulk Property Detectors
    2.10.2  Solute Property Detectors
  References
CHAPTER 3  HIGH PERFORMANCE CAPILLARY ELECTROPHORESIS AND ITS APPLICATION IN PHARMACEUTICAL ANALYSIS
  3.1  Introduction
    3.1.1  History of Development of HPCE
    3.1.2  Advantages of HPCE
    3.1.3  Development of High Performance Capillary Electrophoresis Instrument and Technology
  3.2  CE Instrument and Electroosmotic Flow
    3.2.1  Basic Configuration of HPCE Instrument
    3.2.2  Electroosmotic Flow (EOF)
  3.3  Capillary Zone Electrophoresis
    3.3.1  Separation Principle
    3.3.2  Electrophoretic Velocity and Mobility
    3.3.3  Measurement of Electrophoretic Velocity and Mobility
    3.3.4  Factors on Electrophoretic Mobility and Separation
    3.3.5  Electrophoretic Separation Parameters
    3.3.6  Condition Selection in the CZE
  3.4  Micellar Electrokinetic Capillary Chromatography
    3.4.1  Formation of Micelle
    3.4.2  Characteristics of MEKC
    3.4.3  The Parameters and Factors Affecting the Separation
    3.4.4  Application of MEKC
  3.5  Capillary Isoelectric Focusing
    3.5.1  Principles of CIEF
    3.5.2  Advantage of CIEF
    3.5.3  Application of the CIEF
  3.6  Capillary Gel Electrophoresis
    3.6.1  Separation Mechanism
    3.6.2  Gels Used in the CGE Separation
    3.6.3  CGE for the Protein Molecular Weight Determination
  3.7  Capillary Isotachophoresis
    3.7.1  Conditions Required for CITP
    3.7.2  Characteristics of CITP
    3.7.3  On-line Concentration with CITP
    3.7.4  Application
  3.8  Capillary Electrochromatography
    3.8.1  The Separation Principles and Types of CEC
    3.8.2  Basic Instrumentation of CEC
    3.8.3  Characteristics of CEC
    3.8.4  Commercial CEC Capillaries
    3.8.5  Characteristics of Electroosmotic Flow in CEC
    3.8.6  CEC Column Efficiency
    3.8.7  Applications of CEC
  References
CHAPTER 4  APPLICATION OF SEPARATION TECHNOLOGY IN BIOCHEMICAL DRUG ANALYSIS AND DISEASE DIAGNOSIS
  4.1  Biological Molecules Separation
  4.2  Application of Separation Technology in Disease Diagnosis
    4.2.1  Separation Technology in COVID-19 Prevention and Treatment
    4.2.2  Cancer Treatment
    4.2.3  Diabetes Treatment
  4.3  Problems and Challenges
References
CHAPTER 5  NON-TARGETED SCREENING IN THE ANALYSIS OF COMPLEX SAMPLES WITH UNKNOWN COMPONENTS
  5.1  Targeted Screening and Non-targeted Screening
    5.1.1  Targeted Screening Analysis
    5.1.2  Non-targeted Screening Analysis
  5.2  Separation and Detection Technology in Non-targeted Screening Analysis
    5.2.1  Separation and Identification
    5.2.2  Compound Identification
  5.3  Sample Pretreatment Technologies in the Non-targeted Screening
    5.3.1  "Dilute-and-shoot" Process
    5.3.2  QuEChERS Technology
    5.3.3  Liquid-liquid Extraction
    5.3.4  Solid-phase Extraction in the Sample Pretreatment
  5.4  Novel Materials in Non-targeted Screening Analysis
  5.5  Summary and Prospect
  References
CHAPTER 6  NEW MATERIAL DEVELOPMENT FOR PHARMACEUTICAL SEPARATIONS
  6.1  Ordered Mesoporous Material
    6.1.1  Silica-based Mesoporous Material
    6.1.2  Non-silicon-based Mesoporous Materials
  6.2  Molecularly-imprinted Polymers (MIPs)
  6.3  New Types of Restricted Access Materials
    6.3.1  Characteristic Structure and Function of Restricted Access Materials
    6.3.2  Restricted Access Materials with Molecular Imprinted Polymer Properties
    6.3.3  Synthesis of Restricted Access Material by Controlled/Living Radical Polymerization
  References
QUESTIONS AND PROBLEMS
INDEX